Volta Labs, the company behind the Callisto Sample Prep System, has introduced its DNA Library Preparation Application designed for the Oxford Nanopore Technologies Ligation Sequencing Kit V14 (SQK-LSK114). Created through Oxford Nanopore’s Compatible Products Programme, the new Callisto workflow allows laboratories to perform fully automated, ligation-based library preparation tailored for use with the PromethION sequencing platform.
Performance testing and an independent clinical assessment conducted at University Medical Center Utrecht showed that libraries prepared using Callisto generated as much as 120 gigabases (Gb) of data from a single PromethION flow cell, while achieving diagnostic-level variant detection accuracy with a 99.66% SNV recall rate.
The UMC Utrecht study, which was presented during Advances in Genome Biology and Technology 2026, demonstrated that Callisto’s combined high molecular weight DNA extraction and ligation-based library preparation can support routine whole genome sequencing in workflows designed to meet ISO 15189 laboratory standards.
Bert van der Zwaag explained that reliable automation is critical as the institution moves toward routine clinical whole genome sequencing. He noted that Callisto’s high molecular weight DNA extraction and library preparation capabilities have provided the consistency and throughput necessary to progress toward ISO 15189 accreditation for Oxford Nanopore sequencing, while maintaining variant-calling performance similar to existing short-read sequencing pipelines.
Thomas Bray stated that the results achieved with the Callisto Sample Prep System highlight the capabilities of the PromethION platform when combined with high-quality sample preparation for whole genome sequencing. He also welcomed Volta Labs into Oxford Nanopore’s Compatible Products Programme.